Anti-HIV Activity and Cytotoxicity

Studies on Anti-HIV Activity and Cytotoxicity of Noni Juice
Periyasamy Selvam*, Narayanan Murugesh,2 Myriam Witvrouw 31Arulmigu Kalasalingam College of Pharmacy, Krishnankoil,
626 190, India 2Institute of Pharmacology, Madurai Medical College , Mudurai-625 020, India 3Molecular Medicine, Katholieke Universitiet-Leuven and IRC KULAK, Kapucijnenvoer33, B-3000 Leuven, Flanders , Belgium .

Key words : Morinda citrifolia L, Anti-HIV activity, MT-4 Cells, Cytostatic

Anti HIV Assay: Morinda citrifolia L. was tested for their inhibitory effects against replication of HIV-1 (IIIB) in MT-4 cells (Pauwels, R et al 1988). The MT-4 cells were grown and maintained in RPMI 1640 DM Medium supplemented with 10% (v/v) heat-inactivated Fetal Calf Serum (FCS), 2 mM-glutamine, 0.1% Sodium bicarbonate and 20μg/ml gentamicin (culture medium). Inhibitory effect of Morinda citrifolia on HIV-1 replications was monitored by inhibition of virus-inducted cytopathic effect in MT-4 cells and were estimated by MTT assay. Briefly, 50 μl of HIV-1 (IIIB) (100-300 CCID50) were added to a flat-bottomed microtiter tray with 50 μl of medium containing various concentrations of Morinda citrifolia. MT-4 cells were added at a final concentration of 6 x 105 cells/ml. After 5 days of incubation at 37°C, the number of viable cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny1 tetrazolium bromide (MTT) method.

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